- ET - nm Laser Bandpass Set for mCherry, mKate2, AlexaFluor , Texas Red® - ET - nm Laser Longpass Set for AlexaFluor , mKate2, TurboFP, NirFP - ET - nm Laser Longpass Set for AlexaFluor , DyLight , Atto • Mutiplexable with green, deep red, violet and blue emission dyes, such as GFP, DAPI, Hoechst, HCS LipidTOX ™, and Lysotracker Deep Red. Table 1 Contents and storage Product Catalog No. Amount Concentration Storage Image-iT™ TMRM Reagent (Mitochondrial membrane potential indicator) I 5 × μL μM (X). Jan 10,  · LysoTracker is a group of dyes available in different colors used to stain acidic compartments such as lysosomes. These are membrane permeable weak bases linked to a fluorophore. LysoTracker Red: nm: nm: Lissaminerhodamine: nm: nm: Cy nm: nm: Rhodamine Red-X antibody conjugate pH nm: nm.

ReZolve-L2 in acidic compartments vs Lysotracker

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May 16,  · For Lysotracker Red staining of root, 5-day-old seedlings were applied with Lysotracker Red (10 μg/mL in DMSO, Invitrogen) for 5–10 min and washed out with liquid pollen germination media. May 14,  · Nuclei, mitochondria, and lysosomes were stained with NucBlue, MitoTracker Green, or LysoTracker Red, respectively. Representative fluorescence images of cells. Scale bar = 20 µm. b) Co-localization index of each probe with MitoTracker Green or LysoTracker Red. The index was calculated by dividing the area of overlap between pH probe and. An example of this approach is the application of target-specific small molecule dyes (e.g. MitoTracker Red, LysoTracker Red) for live cell STORM. Structure-specific small molecule dyes used for STORM are included in the Table 1 for the reader’s reference. However, such dyes are generally lipophilic and thus do not target any particular.

In addition, the cells were stained with LysoTracker Red DND (targeting lysosomes; excitation at nanometers and emission at nanometers) and Alexa. chemBlink provides information about CAS # , LysoTracker Red DND 99, molecular formula: C20H24BF2N5O. LysoTracker Red was used as a marker for late endosomes and lysosomes (B, E and H). Plasmid DNA was not colocalized with LysoTracker Red which had a very. Fig. S Validations of lysosomal biogenesis and acidity in zebrafish embryos. (A) Whole-mount double staining with LysoTracker (10 μM, DND; red) and. LysoTracker® 系列探针是对活细胞中的酸性区室进行选择性染色的一类荧光染料，该类探针具有几大重要的特点，1）选择性标记酸性细胞器；2）纳摩尔级（nM）浓度即可有效标记活细胞；3）具有多色探针提供，可根据情况对样品进行多标实验。LysoTracker® 结构上由一个荧光基团和相连的弱碱基构成，可. LysoTracker® Red DND and MitoTracker® Green FM. Bovine pulmonary artery endothelial cells incubated simultaneously with CellTrace™ calcein red-orange AM and MitoTracker® Green FM. Bull sperm. MitoTracker® Green FM and Hoechst Co-localization while using LysoTracker® Deep Red. MitoSOX red, lysotracker red, nile red and BODIPY / staining Living cells were incubated with ™MitoSOX Red ( μM, M, Invitrogen), Lysotracker Red DND (50 nM, L, Invitrogen), Nile Red (1 μM, HY-D, MCE) or BODIPY staining solution (2 μM, GC, GLPBIO) for 15 min at 37 °C in dark. en, cells were washed with. Cells must be imaged live; DO NOT FIX! To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to.

(A) Segmentation of macrophage boundary, bacteria and lysosomes from the images. The raw image of an Mtb-GFP infected cell stained for lysotracker red (I) is. The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. CytoPainter LysoDeep Red. Here we have employed LysoTracker Green (LTG) and Red (LTR) to measure the autophagic process flow cytometrically after induction by chloroquine and rapamycin.

Lysotracker red - May 14,  · Nuclei, mitochondria, and lysosomes were stained with NucBlue, MitoTracker Green, or LysoTracker Red, respectively. Representative fluorescence images of cells. Scale bar = 20 µm. b) Co-localization index of each probe with MitoTracker Green or LysoTracker Red. The index was calculated by dividing the area of overlap between pH probe and.

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