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The resulting fluorescence can be measured using fluorescence imaging, high-content imaging, microplate fluorometry, or flow cytometry. CytoPainter LysoDeep Red. Autophagy participates in the delivery of Mut-PrP to LysoTracker labeled vesicles. followed by incubation with 50 nM LysoTracker Red for 30 min prior to. LysoTracker® Red is a Fluorescent Acidotropic Probe for labeling and tracking acidic organelles in live cells.3, 4 This probe has several important features.

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Fig. S Validations of lysosomal biogenesis and acidity in zebrafish embryos. (A) Whole-mount double staining with LysoTracker (10 μM, DND; red) and. chemBlink provides information about CAS # , LysoTracker Red DND 99, molecular formula: C20H24BF2N5O. LysoTracker™ Deep Red is a deep red-fluorescent dye for labeling and tracking acidic organelles in live cells. The LysoTracker™ probes consist of a.

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LysoTracker and MitoTracker Red are fluorescent probes widely used for viable cell staining of lysosomes and mitochondria, respectively. (A) Segmentation of macrophage boundary, bacteria and lysosomes from the images. The raw image of an Mtb-GFP infected cell stained for lysotracker red (I) is. Fig. S Validations of lysosomal biogenesis and acidity in zebrafish embryos. (A) Whole-mount double staining with LysoTracker (10 μM, DND; red) and.